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  • MAb Technologies | Your source for quality monoclonal and polyclonal antibodies

Norepinephrine Transporter-human (NET17-1)

Norepinephrine Transporter-human  (NET17-1)
Immunohistochemical staining of NET fibers in human cortex.(Image courtesy of Dr. Brian Ciliax) A western blot of SK-N-MC cells transfected with vector or hNET pcDNA which shows the antibody recognizing a more highly glycosylated hNET protein (~80 kD), as well as degraded or lesser glycosylated ...Read more
$275.00 each
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NET-ICCImmunohistochemical staining of NET fibers in human cortex.
(Image courtesy of Dr. Brian Ciliax)

NET blotA western blot of SK-N-MC cells transfected with vector or hNET pcDNA which shows the antibody recognizing a more highly glycosylated hNET protein (~80 kD), as well as degraded or lesser glycosylated protein forms of hNET (~50-55 kD). The image was captured using Alpha Innotech's ChemiImager System. (Picture courtesy of Dr. Gary Miller)

Catalog # NET17-1

Size: 100 ul hybridoma media in glycerol.

Host: Mouse

Known Specificity: Human and non-human primate (does not recognize rodent NET).

Recommended Dilutions:

Immunohistochemistry 1:1,000-2,000

These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.

Source: This mouse monoclonal antibody was raised against a peptide (amino acids 17-33) coupled to KLH by the addition of an N-terminal cysteine. Hybridoma media was obtained from cells grown in a bioreactor.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

References

(All papers reference our antibody)

Blotting:

Zhang, L et al.--Correlation of Somatostatin Receptor-2 Expression with Gallium-68-DOTA-TATE Uptake in Neuroblastoma Xenograft Models.  Contrast Media & Molecular Imaging, 2017.

Straznicky, NE et al.--Norepinephrine transporter expression is inversely associated with glycaemic indices: a pilot study in metabolically diverse persons with overweight and obesity. Obesity Science & Practice, 2016.

Zanzonico, PB et al.--Imaging the Norepinephrine Transporter in Neuroblastoma: A Comparison of [18F]-MFBG and 123I-MIBG. Imaging, Diagnosis, Prognosis, 2014.

Wang et al.--Effects of DSP4 on the Noradrenergic Phenotypes and Its Potential Molecular Mechanisms in SH-SY5Y Cells.  Nerotox Research, 2013.

Mannangatti et al.--Cocaine Up-regulation of the Norepinephrine Transporter Requires Threonine 30 Phosphorylation by p38 Mitogen-activated Protein Kinase.  J Biol Chem, 2011.

Jeannotte et al.--Desipramine Modulation of alpha-, gamma-Synuclein, and the Norepinephrine Transporter in an Animal Model of Depression. Neuropsychopharmacology, 2008.

Jeannotte, AM & Sidhu A--Regulation of the norepinephrine transporter by alpha-synuclein-mediated interactions with microtubules. Eur J Neurosci, 2007.

Mason et al.--Desvenlafaxine succinate identifies novel antagonist binding determinants in the human norepinephrine transporter. J Pharmacol Exp Ther, 2007.

Tong et al.--Marked dissociation between high noradrenaline vs. low noradrenaline transporter levels in human nucleus accumbens. J Neurochemistry, 2007.

Dipace et al.--Amphetamine induces a calcium/calmodulin-dependent protein kinase II-dependent reduction in norepinephrine transporter surface expression linked to changes in syntaxin 1A/transporter complexes. Mol Pharmacol, 2007.

Sogawa et al.--C-terminal region regulates the functional expression of human noradrenaline transporter splice variants. Biochem J, 2007.

Wersinger et al.--Attenuation of the norepinephrine transporter activity and trafficking via interactions with alpha-synuclein. Eur J Neursci, 2006.

Amano et al.--Identification of a novel regulatory mechanism for norepinephrine transporter activity by the IP3 receptor. Eur J Pharmacol, 2006.

Holton et al.--Nonclassical, distinct endocytic signals dictate constitutive and PKC-regulated neurotransmitter transporter internalization. Nature Neurosci, 2005.

Mash et al.--Norepinephrine transporter immunoblotting and radioligand binding in cocaine abusers. J Neurosci Methods, 2005.

Hahn et al.--A mutation in the human norepinephrine transporter gene (SLC6A2) associated with orthostatic intolerance disrupts surface expression of mutant and wild-type transporters. J Neuroscience, 2003.

Sung et al.--A regulated interaction of syntaxin 1A with the antidepressant-sensitive norepinephrine transporter establishes catecholamine clearance capacity. J Neuroscience, 2003.

Bauman, PA & Blakely, RD--Determinants within the C-terminus of the human norepinephrine transporter dictate transporter trafficking, stability and activity. Archives of Biochemistry and Biophysics, 2002.

Zhu et al.--Effects of desipramine treatment on norepinephrine transporter gene expression in the cultured SK-N-BE(2)M17 cells and rat brain tissue. J Neurochemistry, 2002.

Apparsundaram et al.--Trafficking-Dependent and -Independent Pathways of Neurotransmitter Transporter Regulation Differentially Involving p38 Mitogen-Activated Protein Kinase Revealed in Studies of Insulin Modulation of Norepinephrine Transporter in SK-N-SH Cells. J Pharmacol Exp Ther, 2001.

ICC/IHC:

DuBois et al.--Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Childrens' Oncology Group.  Int J Mol Imaging, 2012.

Sogawa et al.--C-terminal region regulates the functional expression of human noradrenaline transporter splice variants. Biochem J, 2007.

Immunoprecipitation:

Wersinger et al.--Attenuation of the norepinephrine transporter activity and trafficking via interactions with alpha-synuclein. Eur J Neursci, 2006.

Mao et al.--Norepinephrine induces endoplasmic reticulum stress and down-regulation of norepinephrine transporter density in PC12 cells via oxidative stress. Am J Physiol Heart Circ Physiol, 2004.

FACS:

Doubrovin et al.--In vivo imaging and quantitation of adoptively transferred human antigen-specific T cells transduced to express a human norepinephrine transporter gene. Cancer Res, 2007.

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