This mouse monoclonal antibody displays punctate staining along fibers in regional patterns consistent with 5-HT fibers. Neurons are detected in the Dorsal/Median Raphe Nucleus.
A western blot of monkey tissue shows regional distribution consistent with published data. Prominent bands are detected at ~92kD and ~60kD.
Size: 50 ul hybridoma media in glycerol.
Known Specificity: Human and non-human primate.
Recommended Dilutions: IHC 1:5,000-10,000
Western blotting 1:7,500-10,000
These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.
Source: This mouse monoclonal antibody was raised against a peptide from the N-terminus coupled to KLH by the addition of an N-terminal cysteine. Hybridoma media was obtained from cells grown in a bioreactor.