A western blot of mouse tissue (50 ug per lane) probed with Fto06-1at 1:2,500. A major band is detected at ~60 kDa.
(Picture courtesy of Dr. Peng Jin)
Catalog # Fto06-1
Size: 50 ul ascites in glycerol.
Known Specificity: Human, mouse and rat. Other species not tested. Antigen shares 100% sequence homology to non-human primate, cow, dog, horse and sheep.
Western blotting 1:2,000-2,500 on tissue
Immunohistochemistry To be determined.
These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.
Source: Both the mouse and rabbit antibody were raised against a peptide (amino acids 6-24) coupled to KLH by the addition of an C-terminal cysteine.