A western blot of recombinant hTNFR1 and hTNFR2 protein (100 ng per lane) probed with TNFR1-5 at 1:1,000. The only detectable band is at ~55 kDa. (Picture courtesy of Dr. Malu Tansey)
Catalog # TNFR1-5
Size: 100ul hybridoma media in glycerol.
Host: Mouse (IgG2a)
Known Specificity: Human.
Mouse and rat - not tested.
Western blotting 1:1,000
Immunohistochemistry To be determined.
These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.
Source: This mouse monoclonal antibody was raised to an extracellular domain using a peptide (amino acids 23-39) coupled to KLH by the addition of an C-terminal cysteine. Hybridoma media was obtained from cells grown in culture media and concentrated by ammonium sulfate.