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Norepinephrine Transporter-mouse (NET05-2)

Norepinephrine Transporter-mouse  (NET05-2)
(click image for larger view with labels) Immunocytochemical staining of NET (NET05-2) in mouse SCG cultures using wt and KO pups for the ganglia harvest.(Image courtesy of Dr. Randy Blakely) Mouse cortex homogenate after a 1000xg spin to eliminate nonhomegnized tissue fragments ofwildtype (WT S1 ...Read more
$275.00 each


  • Description
  • References

NET05-ICC-sm(click image for larger view with labels) Immunocytochemical staining of NET (NET05-2) in mouse SCG cultures using wt and KO pups for the ganglia harvest.
(Image courtesy of Dr. Randy Blakely)

NET05 wt koblot-smMouse cortex homogenate after a 1000xg spin to eliminate nonhomegnized tissue fragments ofwildtype (WT S1) and NET knockout (NET KO S1) tissue.
(Image courtesy of Dr. Randy Blakely)

Catalog # NET05-2

Size: 100 ul hybridoma media in glycerol.

Host: Mouse (IGg2b), clone 2-3B2

Known Specificity: mouse and rat (does not recognize human NET); sequence homology: cow (92%); cat, human, non-human primate (85% ).

Recommended Dilutions:

Western blotting 1:1,000

Immunohistochemistry To be determined.

These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.

Source: This mouse monoclonal antibody was raised against a peptide coupled to KLH by the addition of an C-terminal cysteine. Hybridoma media was obtained from cells grown in culture and concentrated by ammonium sulfate.

NEW recombinant rabbit (Ab02548-23.0) and rat (Ab02548-6.1) norepinephrine transporter (NET05-2) antibody!

 

 

 

 

 

 

 

 

(All papers reference our antibody)

Blotting:

Ye, R et al.--Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters. Frontiers in Synaptic Neuroscience, 2015.

Ayala-Lopez, N et al.--Organic cation transporter 3 contributes to norepinephrine uptake into perivascular adipose tissue. Am J Physiology, Heart Circ Physiology, 2015.

Mannangatti, P et al.--A Role for p38 Mitogen-activated Protein Kinase-mediated Threonine 30-dependent Norepinephrine Transporter Regulation in Cocaine Sensitization and Conditioned Place Preference.  J Biological Chemistry, 2015.

Fentress, HM et al.--Norepinephrine transporter heterozygous knockout mice exhibit altered transport and behavior.  Genes Brain Behav, 2013.

Ma, Y et al.--Extracellular norepinephrine clearance by NET is required for skeletal homeostasis.  J Biol Chem, 2013.

Oaks, AW et al.--Age-Dependent Effects of A53T Alpha-Synuclein on Behavior and Dopaminergic Function.  PLoS One, 2013.

Shirey-Rice, JK et al.--Norepinephrine transporter variant A457P knock-in mice display key features of human postural orthostatic tachycardia syndrome.  Dis Model Mech, 2013.

Arapulisamy, O et al--Regulated Norepinephrine Transporter Interaction with the Neurokinin-1 Receptor Establishes Transporter Subcellular Localization.  J Biol Chem, 2013.

Mannangatti, P et al.--Cocaine Up-regulation of the Norepinephrine Transporter Requires Threonine 30 Phosphorylation by p38 Mitogen-activated Protein Kinase.  J Biol Chem, 2011.

Siuta, MA et al.--Dysregulation of the Norepinephrine Transporter Sustains Cortical Hypodopaminergia and Schizophrenia-Like Behaviors in Neuronal Rictor Null Mice.  PLoS Biology, 2010.

Graham, DR et al--Mice Expressing the A53T Mutant Form of Human AlphaSynuclein Exhibit Hyperactivity and Reduced Anxiety-Like Behavior.  J Neuroscience Research, 2010.

Matthies, HJ et al.--Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.  J Neuroscience, 2010.

Matthies, HJ et al.--Subcellular localization of the antidepressant-sensitive norepinephrine transporter.  BMC Neuroscience, 2009.

ICC/IHC:

Chalermpalanupap, T et al.--Locus Coeruleus Ablation Exacerbates Cognitive Deficits, Neuropathology, and Lethality in P301S Tau Transgenic Mice. J Neuroscience, 2018.

Pierce, MR et al.--Sodium-dependent vitamin C transporter-2 mediates vitamin C transport at the cortical nerve terminal.  J Neuroscience Res, 2015.

Cartier, E et al--Rare Autism-Associated Variants Implicate Syntaxin 1 (STX1 R26Q) Phosphorylation and the Dopamine Transporter (hDAT R51W) in Dopamine Neurotransmission and Behaviors.  EBioMedicine, 2015.

Taylor, TN et al.--Reduced vesicular storage of catecholamines causes progressive degeneration in the locus ceruleus.  Neuropharmacology, 2014.

Ma, Y et al.--Extracellular norepinephrine clearance by NET is required for skeletal homeostasis.  J Biol Chem, 2013.

Erickson, SL et al.--Chronic desipramine treatment alters tyrosine hydroxylase but not norepinephrine transporter immunoreactivity in norepinephrine axons in the rat prefrontal cortex.  Int J Neuropsychopharmacology, 2011.

Matthies, HJ et al.--Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.  J Neuroscience, 2010.

Jardanhazi-Kurutz, D et al.--Induced LC degeneration in APP/PS1 transgenic mice accelerates early cerebral amyloidosis and cognitive deficits.  Neurochem Int, 2010.

Matthies, HJ et al.--Subcellular localization of the antidepressant-sensitive norepinephrine transporter.  BMC Neuroscience, 2009.

Immunoprecipitation:

Mannangatti, P et al.--A Role for p38 Mitogen-activated Protein Kinase-mediated Threonine 30-dependent Norepinephrine Transporter Regulation in Cocaine Sensitization and Conditioned Place Preference.  J Biological Chemistry, 2015.

Arapulisamy, O et al.--Regulated Norepinephrine Transporter Interaction with the Neurokinin-1 Receptor Establishes Transporter Subcellular Localization.  J Biol Chem, 2013.

Matthies, HJ et al.--Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.  J Neuroscience, 2010.

Matthies, HJ et al.--Subcellular localization of the antidepressant-sensitive norepinephrine transporter.  BMC Neuroscience, 2009.

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