This rabbit polyclonal antibody to VGluT1 displays neuropil labeling in rat striatum.
A western blot of rat striatum probed with VGT1-3 (1:6,000) detects a single band at the predicted molecular weight of ~63 kD. Preabsorption of VGT1-3 with the VGT1 peptide blocks detection of the band, but is not affected by preabsorption with an irrelevant peptide (VGT2).
Catalog # VGT1-3
Size: 100 ul rabbit sera in glycerol.
Host: Rabbit
Known Specificity: Rat (Other species not tested. Antigen shares a high degree of sequence homology to human, non-human primate and mouse).
Recommended Dilutions:
Immunohistochemistry 1:5,000-15,000
Western blotting 1:6,000
These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.
Source: This rabbit polyclonal antibody was raised against a c-terminal peptide coupled to KLH by the addition of an N-terminal cysteine.