mab_secondary_slides Vesicular Glutamate Transporter 1 (VGT1-3) - MAb Technologies | monoclonal antibodies to transporter proteins

Vesicular Glutamate Transporter 1 (VGT1-3)

Vesicular Glutamate Transporter 1 (VGT1-3)
This rabbit polyclonal antibody to VGluT1 displays neuropil labeling in rat striatum. A western blot of rat striatum probed with VGT1-3 (1:6,000) detects a single band at the predicted molecular weight of ~63 kD. Preabsorption of VGT1-3 with the VGT1 peptide blocks detection of the band, but is n ...Read more
$275.00 each


  • Description
  • References

vgt1This rabbit polyclonal antibody to VGluT1 displays neuropil labeling in rat striatum.

rVGT1-3-blotA western blot of rat striatum probed with VGT1-3 (1:6,000) detects a single band at the predicted molecular weight of ~63 kD. Preabsorption of VGT1-3 with the VGT1 peptide blocks detection of the band, but is not affected by preabsorption with an irrelevant peptide (VGT2).

Catalog # VGT1-3

Size: 100 ul rabbit sera in glycerol.

Host: Rabbit

Known Specificity: Rat (Other species not tested. Antigen shares a high degree of sequence homology to human, non-human primate and mouse).

Recommended Dilutions:

Immunohistochemistry 1:5,000-15,000

Western blotting 1:6,000

These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.

Source: This rabbit polyclonal antibody was raised against a c-terminal peptide coupled to KLH by the addition of an N-terminal cysteine.

 

 

 

 

 

 

 

 

(All papers reference our antibody)

Blotting:

Raju et al.--Differential synaptic plasticity of the corticostriatal and thalamostriatal systems in an MPTP-treated monkey model of parkinsonism. Eur J Neurosci, 2008.

Mark et al--Dynamic changes in vesicular glutamate transporter 1 function and expression related to methamphetamine-induced glutamate release. J Neuroscience, 2007.

Raudensky J and Yamamoto BK--Effects of chronic unpredictable stress and methamphetamine on hippocampal glutamate function. Brain Res, 2007.

Raju et al.--Differential synaptology of vGluT2-containing thalamostriatal afferents between the patch and matrix compartments in rats. J Comp. Neuro, 2006.

Immunocytochemistry (ICC):

Mathur BN and Deutch AY--Rat meningeal and brain microvasculature pericytes co-express the vesicular glutamate transporters 2 and 3. Neurosci Lett, 2008.

Immunohistrochemistry (IHC):

Fauner M et al.--Excitatory and Inhibitory innervation of the mouse orocacial motor nuclei: A sterological study. J Comp Neuro, 2016.

Balaram et al.--VGLUT1 mRNA and protein expression in the visual system of prosimian galagos (Otolemur garnetti).   Eye Brain, 2011.

Villalba R et al.--Differential Structural Plasticity of Corticostriatal and Thalamostriatal Axo-Spinous Synapses in MPTP-Treated Parkinsonian Monkeys. J Comp Neurol, 2011.

Doig NM et al.--Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum.  J Neuro, 2010.

Raju et al.--Differential synaptology of vGluT2-containing thalamostriatal afferents between the patch and matrix compartments in rats. J Comp. Neuro, 2006.

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