A western blot of HEK293 cells transfected and untransfected with mouse Fto cDNA. A single band is detected at ~60 kDa when probed with Fto06-3 at 1:1000.
(Picture courtesy of Dr. Peng Jin)
Catalog # Fto06-3
Size: 100 ul rabbit sera in glycerol.
Known Specificity: Human, mouse and rat Other species not tested. Antigen shares 100% sequence homology to non-human primate, cow, dog, horse and sheep.
Western blotting 1:500-1,000 on transfected cells.
Immunohistochemistry Does not work.
These working conditions are provided as a suggestion only. Each user should determine optimal conditions for his or her own experiment.
Source: Both the mouse and rabbit antibody were raised against a peptide (amino acids 6-24) coupled to KLH by the addition of an C-terminal cysteine.